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Exposure of MCF-7 cells to 0.1 μM DOX for 2 days induced a differentiation-like phenotype with prominent perinuclear autocatalytic vacuoles, abundant filamentous material, and irregular microvilli at the cell surface.
There are also other instances where lower expression of a ribosomal protein triggers differentiation-like responses.
Differentiation-like morphological changes in HCT-116 cells upon miR-24 transfection was quantitated by light-microscopy [38].
This loss of proliferative potential occurs by activation of a differentiation-like program that causes stochastic G1 arrest, and is accompanied by characteristic morphological changes (reviewed in [4]).
Our results indicate that alteration of the corneal basement membrane induces a de-differentiation-like phenotype in corneal basal epithelial cells.
The differentiation-like morphological change was not observed in HCT-116 cells transfected with siDHFR and Oligofectamine alone (Cont) (Fig. 4B).
MiR-24 overexpression down regulated DHFR levels by approximately six fold as compared to Oligofectamine alone transfected cells (Fig. 4A), and conferred a morphological change resembling a differentiation-like phenotype in a colorectal cancer cell line (HCT-116-wt-p53).
Moreover, by day 9, cells attained myelocyte morphology with oval-shaped nucleus with obvious differentiation-like morphology even in G-CSF-untreated mock transfected cells in addition to siE6AP and E6AP-C843A-transfected E6AP-C843A-transfected E6AP-C843A-transfected
The MNase digestion assay using fixed chromatin confirmed that after day 6 of differentiation, like TSCs, dTSCs also formed nucleosomal structures (Fig. 4c).
Elements critical for tissue differentiation, like extracellular stimuli, adhesion and cell shape properties, and transcription factors all contribute to the modulation of gene expression and thus, are likely to impinge on the nuclear mechanisms of epigenetic gene expression control.
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