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The HL-60 cells were differentiated by adding 1.25% dimethylsulfoxide (DMSO) to the cell suspension, and differentiation was evaluated by observing morphological changes evident 6 7 days after DMSO addition.
Fb differentiation was evaluated by counting the number of cells positive for either F-actin or α-SMA stress fibers.
Cell osteogenic differentiation was evaluated by alkaline phosphatase (ALP) activity analysis, Alizarin Red S staining and real-time PCR assay.
Cell viability was quantified using a tetrazolium-based assay and osteogenic differentiation was evaluated by both alkaline-phosphatase (ALP) histochemistry and osteocalcin mRNA analysis.
Alveolar bone loss was estimated by morphometry, gingival blood flow was measured using laser Doppler flowmetry, and osteoclast differentiation was evaluated by tartrate-resistant acid phosphatase staining.
Cell viability was analyzed by MTT and osteogenic differentiation was evaluated by alkaline phosphatase (ALP) activity assay, Alizarin red S dye, real time-polymerase chain reaction (RT-PCR) and Western blot analysis.
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Population differentiation is evaluated by the fixation index F ST 39.
The effects of SCPP on cells' proliferation and differentiation were evaluated by MTT and ALP activity assay.
Re-differentiation was evaluated by counting vesicular protoscoleces.
Differentiation was evaluated at different time points by osteoblast differentiation associated gene expression, alkaline phosphatase (ALP) activity, histochemical staining for mineralized matrix and calcium quantification.
The pathological grade of tumor differentiation was evaluated according to the criteria proposed by Edmonson and Steiner.
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