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Caco-2 cells were used for toxicity evaluations and their differentiation was evaluated with the measurement of transepithelial resistance.
Histological differentiation was evaluated, as poorly differentiated carcinomas are known to have a high-risk of recurrence or metastasis.
The HL-60 cells were differentiated by adding 1.25% dimethylsulfoxide (DMSO) to the cell suspension, and differentiation was evaluated by observing morphological changes evident 6 7 days after DMSO addition.
The pathological grade of tumor differentiation was evaluated according to the criteria proposed by Edmonson and Steiner.
Fb differentiation was evaluated by counting the number of cells positive for either F-actin or α-SMA stress fibers.
The role of Wnt agonists/antagonists as mediators of androgen inhibition of DPC-induced HFSC differentiation was evaluated.
Cell osteogenic differentiation was evaluated by alkaline phosphatase (ALP) activity analysis, Alizarin Red S staining and real-time PCR assay.
Transcription factor expression levels in the CNS were assessed using real-time PCR; T cell differentiation was evaluated via flow cytometry.
Cell viability was quantified using a tetrazolium-based assay and osteogenic differentiation was evaluated by both alkaline-phosphatase (ALP) histochemistry and osteocalcin mRNA analysis.
Alveolar bone loss was estimated by morphometry, gingival blood flow was measured using laser Doppler flowmetry, and osteoclast differentiation was evaluated by tartrate-resistant acid phosphatase staining.
Differentiation was evaluated at different time points by osteoblast differentiation associated gene expression, alkaline phosphatase (ALP) activity, histochemical staining for mineralized matrix and calcium quantification.
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