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Differentiation was continued until day 8.
In one experiment the differentiation was continued until day 30 to induce an osteocyte-like state of terminal differentiation.
The cells were exposed to TSA for 1, 2, or 3 days, before the drug was removed, and differentiation was continued until DoD6.
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Twenty-four hours after the transfection, the growth medium was replaced by differentiation medium, and the incubation was continued for an additional 96 h.
"The question is whether or not it should be continued with this differentiation between the states.
Differentiation was initiated and continued as for StdD protocol.
Differentiation was allowed to continue for 3 5 days until myotubes were clearly visible.
Differentiation was allowed to continue for 5 9 days until myotubes were clearly visible, and used for the experiments described in results.
"The bombing is continuing and the fighting on the streets is still ongoing and there is no differentiation between civilians and armed people on the streets".
Differentiation was initiated 48 h after of siRNA transfection and continued for 2 weeks.
Differentiation was induced 48 h after the start of the infection, and continued for an additional 24 72 h.
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