The differentiation status was checked on day 4 by intracellular cytokine staining and 2 × 10 cytokine positive T cells were injected i.v. into Rag1 -/- recipient mice, which had been infected with VV-Ova one day prior to T cell transfer.
Differentiation of Caco-2 cells was checked on days 8, 15, 21 by determining the alkaline phosphatase activity using the AKP assay kit, a brush border enzyme marker [15, 25].
The expression of sarcomeric myosin during myogenic differentiation (days 0, 1, 4 and 8) was checked by means of real time RT-PCR (Additional file 1d), and the results indicated that both the control and FSHD muscle cells were undergoing correct myogenic differentiation and had comparable differentiation properties.
Normality was tested using the Shapiro-Wilk test, and significant differentiation between pairs of continental groups within species was checked for by applying pairwise non-parametric Wilcoxon tests.
Herein, influence of strontium on the proliferation and differentiation of cementoblasts were checked in detail.
Roles of other filtrated miRNAs in MSC neuronal differentiation are also waited to be checked in the future.
Twenty-four hours after plating, cells were checked for confluence and differentiated using the commercially available Differentiation Medium DMM, Zen-Bio, Inc).
The corresponding genes are potential differentiation markers that can be useful in developmental studies and can easily be checked by in situ hybridization on embryos.
Osteoclast differentiation was then evaluated by microscopy checking for the expression of the osteoclast specific phosphatase TRAP and the presence of multiple nuclei (≥3).
Different gene markers that are important for brain and retina formation and neuronal differentiation were used to check the phenotypic changes after knockdown of zfPRL2 by morpholino (MO) in zebrafish embryos (Table S2).
