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Therefore, in addition to heritage of its ecological wisdom, syndrome differentiation treatment should be used.
Nodule formation and calcification were strongly increased by the differentiation treatment, especially by dexamethasone.
The phenotype of the macrophages in these protocols varies with the differentiation treatment and duration, reflecting differences in gene transcription [11], [12].
Additional File 3, Figure S1A shows cells immediately post-isolation and three days post differentiation treatment.
We subjected total RNA extracted from THP-1 cells with and without differentiation treatment to quantitative analysis of mRNA expression of IL12B and IFNα.
After 20 days of differentiation treatment, the culture medium was removed, and cells were washed twice with phosphate buffer saline (PBS) and fixed with 4% formalin/PBS.
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HL60 and NB4 cells are distinguished by their marked sensibility to differentiation treatments and both can be triggered to terminally differentiate into granulocytes upon exposure to retinoic acid (RA), whereas HL60 can also differentiate in monocytes when treated with 1,25-dihydroxyvitamin D3 (D3).
For differentiation treatments, cells were cultured as above but without LIF, and supplemented with one of the differentiation agents.
In the other differentiation treatments, the effect was similar between the two genes, with DMSO treatment yielding the strongest response.
Expression levels of all five TUs dropped dramatically during three distinct chemically induced differentiation treatments of ES cells in culture.
The effect was common to all three differentiation treatments tested, indicating that it was not a chemical-specific response, but rather a reproducible outcome of differentiation.
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