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The concentration-dependent effects on development were reflected in the significance of deviation of flusilazole-exposed cultures from this transcriptomic-based differentiation track.
This wrong differentiation track continued to deviate more and more from control cells, also in the absence of the drugs.
However, an altered expression of only two NEP markers is not sufficient to answer the question, if we observe really a changed differentiation track.
Therefore, we conclude that long- but not short-term treatment indeed changes the gene expression pattern toward a wrong (non neuronal) differentiation track.
It may be argued that T6d contains two types of regulated genes: PS altered because of an overall altered differentiation track and PS altered because of the continued presence of the drug.
A hypothetical linear sequential differentiation track that would flip back and forth with respect to CD24 expression and appear as a hidden subpopulation in a majority of cells from another differentiation stage could explain this discrepancy.
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These algorithms work to push progenitors and their transitional intermediates through a series of railroad-station-like switching decisions to regulate flux along specific differentiation tracks.
The PS of T4d that overlapped with T6d and MP pointed already to disturbed differentiation: the oGOs among these down-regulated PS indicated a defect in forebrain development (Fig. S7B) and the up-regulated PS pointed again to an increase in unwanted differentiation tracks (Tab. S4).
Differentiation of track access charges to avoid overloaded links.
Several recent publications have described the use of Raman spectroscopy to determine cell viability, to identify general markers of cell differentiation, to track differentiation to an osteoblastic phenotype and to elucidate changes in extracellular matrix calcification and/or mineralisation concomitant with osteoblastic differentiation.
Differentiation was tracked by determining the share of differentiated and cell cycle-arrested adherent cells and the share of non-differentiated proliferating suspended cells at defined points from 24 h up to 72 h after addition of PMA and/or WIN55212-2 by FACS counting.
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