Parameters important for sperm production are inferred from sensitivity analysis; they include the differentiation time of differentiating spermatogonia and pachytenes, and the division time of diplotenes and secondary spermatocytes.
The differentiation time of either differentiating spermatogonia or pachytenes has negative effects on their respective downstream cells.
We tested all parameters and found that variations in each of six parameters – initial number and division of spermatogonial stem cell, differentiation time, division time, and lifespan of differentiating spermatogonium, and differentiation time of preleptotene – stop the transition to differentiating spermatogonia leading to the VAD phenotype (supplementary material Table S3).
The differentiation time negatively affects the population size of differentiating spermatogonia, but positively correlates with the population size of leptotenes and zygotenes, respectively.
In contrast to Runx2, which increased slightly during the differentiation time course, Sox9 and MyoD were highest early (day 3) and progressively decreased thereafter.
Fluorescence readings of differentiating cells decreased in intensity as a function of differentiation time, which corresponds to more negative membrane potential, or hyperpolarization.
GETPrime primers were able to differentiate both Ubtf splice forms at the two selected differentiation time points and did so in quantitative fashion in that the sum of the individual transcript amounts matched the overall gene expression amount.
LincRNA signatures in a differentiation time course.
Gene flow and differentiation time of Sini.
Error bars indicate the standard deviation for 3 biological replicates at each differentiation time point.
Both miRNA expression was low 2 days after differentiation, time point when Col25a1 transcripts were the most abundant.
