Exact(16)
The differentiation study of oral fibroblast is important for oral and maxillofacial tissue engineering.
Neuro2a is a mouse neural crest-derived cell line that has been widely used as an experimental model for neuronal differentiation study.
For odontogenic differentiation study in vitro, alkaline phosphatase activity quantification, reverse transcription polymerase chain reaction, scanning electron microscopy, von Kossa staining and calcium content quantification were carried out.
In particular, the cell differentiation study showed that the 1 3 nanopatterned substratum (width to spacing ratio, defined by the ridge width of 1 3) exhibited the highest bone mineralization compared to other nanopatterned substrata and the flat substratum.
Notably, the RPE differentiation protocols did not rely on a sphere-formation step during culture, suggesting that the apparent inequality in differentiation potential demonstrated in the NPC differentiation study may have resulted from differences in ECM proteins and was thus an artifact of the protocol.
Floating cultivation of lineage-negative hematopoietic progenitors from bone marrow in an optimized cytokine cocktail (FLT3-L, IL-3, IL-6, and SCF) led to a stable log-scale proliferation of these cells, and a subsequent differentiation study using IL-4/GM-CSF revealed that 3-weeks of expansion was optimal to produce CD11b+/CD11c+ DC-like cells.
Similar(44)
Clonal differentiation studies revealed that CXCR6+ cells within the αLP population differentiate into all ILC lineages but not T- and B-cells.
Biocompatibility studies, namely metabolic activity, mineralization and differentiation studies were conducted with human osteoblastic cell line SAOS-2.
We see multiple applications for this novel system in hepatocyte maintenance and stem cell differentiation studies.
In differentiation studies, the NF scaffolds displayed more uniform matrix and mineral production throughout.
Differentiation studies showed an increase in osteoblast differentiation on fibronectin substrates while collagen substrates lacked osteogenic differentiation.
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