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Our data helped identifying gene sets characterizing the sequential differentiation steps from stem cells to fully differentiated adipocytes.
From the characterization of more than 6,000 proteins, 27,000 transcripts, and 15,000 differentially methylated regions (DMRs), we identified coordinated changes associated with early differentiation steps.
This review covers the differentiation steps between HSCs and committed T cell progenitors within the thymus.
Lines representing early endothelial differentiation steps were selected: MAgEC10.5 and MagEC11.5.
Skeletal muscle undergoes complicated differentiation steps that include cell-cycle arrest, cell fusion, and maturation, which are controlled through sequential expression of transcription factors.
We have previously shown that in vitro differentiation of murine embryonic stem (ES) cells via embryoid bodies (EBs) recapitulates the cellular differentiation steps of chondrogenesis.
Finally, the proposed biosensor was successfully applied for detection of caspase-3 activity during hematopoietic stem cell differentiation steps as a real sample.
Our data indicate that N-cadherin and cadherin-11 were expressed in undifferentiated DBSCs and their expression underwent changes during the osteogenic process (decreasing and increasing respectively), while expression of E-cadherin and P-cadherin was very low in DBSCs and did not change during the differentiation steps.
Nevertheless, these pathologic cells may share differentiation steps with osteoblasts at the molecular level.
Other beta cell markers IAPP and IGRP showed an increase with the differentiation steps.
All differentiation steps were performed in hypoxic conditions (5% O2) in a humidified incubator at 37 °C.
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