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From experiments using 12-o-tetradecanoylphorbol-13-acetate 12-o-tetradecanoylphorbol-13-acetate 12-o-tetradecanoylphorbol-13-acetate 12-o-tetradecanoylphorbol-13-acetate 12-o-tetradecanoylphorbol-13-acetatees is calcium- and differentransformingific.
The occurrence of LOCKs is differentiation specific: there are more LOCKs in differentiated cells, and genes contained in the LOCKs tend to be repressed during differentiation.
Lamin A/C dissociated from the promoter regions of the differentiation specific MyoD, myogenin and muscle creatine kinase genes when myoblasts were induced to differentiate.
Concomitantly, mCherry expression was slightly reduced in the cells, reflecting expression of one or more of the differentiation specific miRNAs miR-223, miR-155, or miR-142-3p miR-142-3p miR-142-3p
Furthermore, the addition of these factors to differentiation specific media instead of serum was enough to induce either osteogenesis or adipogenesis.
Among these colonies, approximately 90% were EGFP negative and mCherry positive, indicating expression of miR-302a/d and no expression of the differentiation specific miRNAs, suggesting a partially reprogrammed state for these transformed cells as previously reported [19], [28], [29].
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FDD identified 18 candidate bands that specifically appeared during the soldier differentiation (differentiation-specific) and two bands that appeared after the completion of differentiation (soldier-specific).
Upon addition of adipogenic or osteogenic factors to MSCM (see Additional file 2E) or M5, hAMSCs differentiated into cells expressing adipocytes and osteocytes markers, respectively, as depicted by adipocyte differentiation-specific Oil red O and osteocyte differentiation-specific AP staining.
Immunophenotyping of acute and chronic leukaemias has revealed many lineage- and differentiation-specific antigens.
This TCDD-induced effect on differentiation-specific proteins results in profound changes in the tissue architecture.
Furthermore, TCDD-treated organotypic cultures exhibit aberrant distribution of several differentiation-specific protein markers.
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