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Then, the cells were grown in several conditions: serum-starvation medium, neural differentiation medium, and expansion medium, as described under the GFAP+NNP growth and differentiation section.
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For the assessment of chondrogenic differentiation, sections of pellets were stained against collagen type II.
SSR markers with high cross-species transferability and high genetic diversity were selected for phylogeny analysis, where the results showed a complex genetic architecture among different sections as well as wide genetic differentiation within section Dendrobium.
As we stated in the "Vertical differentiation model" section, the vertical differentiation can have dramatic effects on the equilibrium because it may lead to big changes.
These data suggest that FGF2 controls the cell-cycle machinery/survival pathways as soon as the level of FGF2 is increased to induce cellular differentiation (see section below: FGF2-induced miRNAs are key major regulators of cell-cycle arrest).
Although major emphasis has been placed on the role of matrix chemistry and configuration in hepatocyte culture and differentiation ﹛see section "Liver biomatrix"), more than a dozen soluble signals have been identified that change qualitatively and quantitatively with differentiation (Wang et al., 2010b; Turner et al., 2011).
To determine whether the BrdU+ cells differentiated, sections were co-labeled with markers of differentiation.
To provide biochemical evidence for altered sebocyte differentiation, histological sections of skin of 4OHT-treated αffβff and 4OHT-treated IKOαβ mice were subjected to Oil Red O staining.
To assess colonocyte differentiation, frozen sections were stained by the "alcian blue-periodic acid Schiff" method to detect mucin, followed by methyl green staining to detect nuclei (AB-PAS-MG) [57].
Out of this subset, a total of 1606 genes could be identified as significantly differentially expressed after induction of differentiation (see Methods section, Microarray data analysis; see Additional file 2).
In the Section 'Differentiation in HR management within business incubators', we will try to define the HR management modes specific to each type of incubator, in the form of proposals.
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