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Amazingly, in spite of low total overlap for three datasets (grey zone, Figure 6B), all normal mouse populations designated as stem cell-like obtained a higher stemness score than differentiation score (P<0.002, binomial test), and four of the datasets scored at least two standard deviations above randomly simulated datasets (Figure 6B).
As expected, mammospheres showed a low differentiation score close to the MaSC profile.
The difference of the two projected positions of each sample along the MaSC → pL → mL axis is referred to as the differentiation score.
Our previously described differentiation score predictor was based on genomic data of the MaSC/BiP-, pL- and mL-enriched subpopulations of Lim's et al. [ 9] (i.e., training set).
Increasingly "undifferentiated" breast cancer cells [as quantitatively measured by a Differentiation Score predictor (DS)] tend to express stem cell signatures and preferentially metastasize to the brain and lung.
Recently, a genomic method to quantify breast epithelial cell differentiation status, known as the Differentiation Score (DS) predictor [ 6] was developed.
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Accordingly, the histological grade of the tumor corresponded to grade-2 (tumor differentiation: score-2; mitotic counts: score-2; tumor necrosis: score-1) following to the French National Federation of Cancer Centers FNCLCCC) grading system [ 12].
Interestingly, low "Differentiation Scores," or high expression of the aforementioned signatures, further predicted for brain and lung metastases.
No distinct differences in the adipogenic differentiation scores were noted between di-MSCs and ex-MSCs, suggesting a similar adipogenic differentiation potential (Table 4).
Out of 57 patients, 27 had normal temperature differentiation scores and 9 could not tell the temperatures apart in the differences set on the measuring device.
The prostatectomy specimens were examined by an experienced uro-genito-pathologist assessing pathological stage (pT) and tumor differentiation scored according to Gleason.
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