Sentence examples for differentiation passage from inspiring English sources

For analysis of neuronal or astrocyte differentiation, passage 5 cells were switched to differentiation media (same as proliferation media except with 2% FCS and no EGF or bFGF) and let proceed for the specified time.

For differentiation assays, passage 4 to passage 7 cells were seeded in 24 or 12 well plates at 5 k/cm, 25 k/cm, 85 k cells/cm, in micromasses (400 k cells in a 20 µl droplet), or on patterned fibronectin substrates; all in growth medium (DMEM + 10% FBS (Atlanta Biologicals, Lawrenceville, GA, USA).

We also assessed neuronal differentiation in passage 19 NSC derived from H9 by intracellular FACS (Fig. S4E).

Again, roughly three quarters of the colonies showed the three-lineage potential and thus no appreciable decrease in neuronal differentiation with passage number.

The current data gives the impression that the overshooting Oct4 expression is correlated with the strong spontaneous differentiation on passage 11 and declines afterwards, but this observation needs further analysis.

Cells were used for differentiation at passage 3 5.

To induce osteogenic and adipogenic differentiation, ADSCs (passage 3) were grown to approximately 90% confluence; then the medium was replaced into adipogenic or osteogenic differentiation medium.

For epithelial differentiation, ASCs (passage 3) were cultured with ATRA (Sigma-Aldrich) at a final concentration of 5  μM as previously described.

In this context, trans-differentiation studies have documented that reprogramming somatic cells directly to a cell of another somatic fate involves de-differentiation and passage through a less differentiated progenitor state (Xie et al, 2004) (Fig 3).

To begin differentiation, cells (passages two through four) were switched to either osteogenic or adipogenic differentiation media and normoxic conditions.

All iPSCs were used for differentiation at passages from 30 to 40.