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The study, the first to follow heart-cell differentiation over time in such detail, could help scientists better understand how particular mutations can lead to congenital heart defects.
Instability, by contrast, indicates divergent evolution, increasing differentiation over time, and the persistence and growth of disturbance effects and initial variations.
"It's basically watching differentiation over time in a dish, and being able to take snapshots of that and put it all together to try to understand how the complex process of cardiac commitment is regulated," Boyer says.
Specifically, we demonstrate the ability of the systems to efficiently encapsulate and release rAAV vectors in a sustained, controlled manner for the effective transduction of hMSCs (up to 80%) without deleterious effects on cell viability (up to 100%) or on their potential for chondrogenic differentiation over time (up to 21 days).
In scenario B, the shortest distance of the hornet to the nest surface (dxz) and its differentiation over time that defines the distance velocity vdxz = Δdxz/Δt were determined.
Furthermore, large population sizes (inferred from sampling success), lack of population differentiation over time or among sites, and evidence that cryptic species were not sampled in this study make related explanations for linkage disequilibrium in T. marchalianum unlikely.
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These observations are consistent with the notion that SCF-R+ cells may constitute an early proliferative LC precursor pool that can quickly differentiate into SCF-R− cells with maintained LC differentiation ability but reduced proliferation capacity, resulting in an ultimate loss of differentiation capacity over time.
CGN, however, require at least 7 days for differentiation and over time cells tend to cluster and loose adherence to the glass substrate.
IL-12 may be produced only early during vaccine priming and programs the entire effector differentiation pathway over time.
One objective of our analysis is to understand the evolution of the differentiation process over time, in particular the impact of different stimulants on this process.
TGF-beta dependent induction of EMT via SMADs pathway increased its significance during differentiation, with, over time, an increase of down-regulated genes (i.e., occludin, fibronectin).
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