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Furthermore, differentiation of the four constructs and their dimensions is difficult; this differentiation depends on the constructs' fundamental definitions and is therefore not selective.
Therefore, a Taqman real-time RT-PCR assay for rapid differentiation of the four major H5N1 clades detected in Vietnam was developed.
The assay was optimized and evaluated using circulating clades of H5N1 collected in Vietnam from 2007 to 2012 and shown to be both sensitive and specific for the differentiation of the four H5N1 clades.
The cDNA chip was designed for detection and differentiation of the four species of selected cucurbit-infecting tobamoviruses [target viruses: Cucumber green mottle mosaic virus (CGMMV); Cucumber fruit mottle mosaic virus (CFMMV); Kyuri green mottle mosaic virus (KGMMV); and Zucchini green mottle mosaic virus (ZGMMV)].
Duplex and multiplex PCR enabled the simultaneous detection and differentiation of the four CMBs, namely ACMV (940 bp), EACMCV (435 bp), EACMMV (504 bp) and EACMZV (260 bp) in single and mixed infections, and sequencing results confirmed virus identities according to the respective published sequences of begomovirus species.
Detailed methods for the differentiation of the four seasons have been described elsewhere (Harley et al. 2009).
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Of late the increase of travel and other inter-communication between England and America has tended to halt the differentiation of the two dialects.
We discuss the physiological and developmental differentiation of the two rice PORs.
The others are its applicability on a large number of sites and a differentiation of the three varieties.
This effect was an underlying reason for the sensitivity differentiation of the two indices and further complicated the characterization of changes in SOC.
Taken together, our findings indicate that the functional differentiation of the two rice PORs is closely associated with maintaining the photosynthetic activity of leaves until senescence.
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