To evaluate the differentiation pattern of spheres, the spheres were dissociated into single cells and plated on to Permanox chamber slides (Lab-Tek, Nunc) at a density of 100 cells per well.
We performed the same assay for the differentiation of tertiary spheres that had been generated from secondary sphere-like colonies.
At the same time, in insisting that the emancipated society must be capable of presenting itself as a totality for its subjects, Lukács is unable to discover any resources for progress in the differentiation of social spheres (Arato and Breines 1979: 155).
Furthermore, mouse neurospheres grown in the conditioned media from JEV-activated microglia exhibit arrest in both proliferation and differentiation of the spheres compared to conditioned media from control microglia.
The differentiation of melanoma spheres was induced by the withdrawal of FGF basic and EGF.
In agreement with this finding, we reproducibly observed a differentiation of colon tumour spheres on exposure to serum-containing conditions.
To favour cell attachment and differentiation of PANC-1 spheres, cells were incubated for 10 days at 37°C in CSM without EGF/FGF but with the addition of 1% FBS [referred to as DM (differentiation medium)].
To obtain differentiation of lung cancer sphere-forming cells, stem cell medium was replaced for 3 days with Bronchial Epithelial Cell Growth Medium (Cambrex, East Rutherford, NJ, USA) in tissue culture-treated flasks, to allow cell attachment and differentiation.
In order to obtain differentiation of lung cancer sphere-forming cells, stem cell medium was replaced with bronchial epithelial cell growth medium (BEGM, Lonza, East Rutherford, NJ, USA) in tissue culture-treated flasks to allow cell attachment and differentiation.
To achieve the in vitro differentiation of BCSCs, dissociated sphere cells were cultured in DMEM for the indicated times (3.5 or 7 days) supplemented with 10% fetal bovine serum (FBS) in adherent conditions.
