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The repeat lengths varied among different iPSC lines and changed during neuronal differentiation of a given iPSC line.
In some cases, however, differentiation of a given neuron is likely to depend on specific intercellular signals that primary cultures will not provide.
The determination and differentiation of a given cell within a tissue is the culmination of the expression of many gene products and their subsequent intra- and intercellular signaling events.
If the posterior distribution of alpha deviates from zero, or 95% of the distribution is positive, diversifying selection may have affected the differentiation of a given locus (high- FST outlier).
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amova tests the partitioning of molecular variation among predefined subpopulations and yields fixation indices (FST values) which describe the extent of genetic differentiation for a given level of population subdivision.
As a whole, immunodetection of different markers demonstrate that the SVZ-derived NS assay is a highly heterogeneous system containing different cell types, and contains cells at different stages along the differentiation pathway of a given neural lineage.
Further selective use of miRNA targets would be predicted to preferentially suppress expression in specific lineages or specific stages of differentiation within a given lineage.
To achieve this capability, the team's approach leverages the entire complement of human transcription factors (TFs), the proteins that directly regulate the expression of genes required for the differentiation and function of a given cell type.
Therefore, an approach based on the average FST in our data seems to be more accurate, in the sense that the average FST of a gene is a better proxy of the amount of genetic differentiation at a given locus.
G'st, a standardized measure of genetic differentiation that represents the proportion of the maximum differentiation possible for a given level of subpopulation homozygosity, was calculated using Hedrick's [35] equation 4b with values from FSTAT 2.9.3 [36].
Therefore, any measure of genetic differentiation calculated for a given SNP may be correlated with the signal from nearby SNPs, if in LD.
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