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As the two NPC lines were initially generated by two different induction protocols, we needed to distinguish whether the differential response was due to the differentiation methods used or their derivation from two different hESC lines.
All isolates were differentiated to the level of species by routine differentiation methods.
Cells were maintained and differentiated in monolayer colonies, as opposed to embryoid body differentiation methods, to facilitate imaging.
We found no marked differences between differentiation methods and decided to perform further experiments with macrophages differentiated with M-CSF and FCS.
The design sensitivities are computed efficiently via analytical differentiation methods.
Refining the differentiation methods can enrich for subtype-specific hiPSC-ECs with functional benefits of enhancing neovascularization.
Similar(19)
We perform numerical experiments to compare these methods with the second-order backward differentiation method, commonly recommended for ordinary differential equations with constraints.
The proposed formulation is based on the implicit differentiation method (IDM), where the boundary integral equations are differentiated analytically with respect to the design variables.
To differentiate the cells, a slight modification of directed cardiac differentiation method was used as previously described [ 30].
S.J.Y., R.M.M. and S.P.P. developed the differentiation method.
Rhesus cardiomyocytes derived by our stepwise differentiation method express signature cardiac markers and show normal electrochemical coupling.
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