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This classification revealed previously unrecognized tissue differentiation lines (adipocyte, fibroblastic, smooth-muscle) and was reproduced in paraffin specimens.
To further demonstrate the molecular basis of reclassifying MFH, we examined whether MFH samples overexpressed genes associated with their predicted differentiation lines.
These findings, taken together, suggest that our proposed reclassification is not merely a mathematical model function; rather it is tracking real underlying molecular sarcoma "phenotypes" based on tissue differentiation lines.
There was also a subset of non-overlapping miRNAs (Table 2) which may be more specific to the different sarcoma differentiation lines.
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Besides, most cells in the mature body are at the very end of the differentiation line, while some cells - stem cells - halt the differentiation process, divide and can give rise to more cells of their own type.
Considering Block 2 alone, there was a similarly strong differentiation among lines in aggression, with the selected Agg line topping the hierarchy, and the unselected Neut line showing the least aggression (n = 405, DF = 9, χ2 = 40.654, P<0.0001).
Only 9.7% of genetic differentiation among lines was explained by grouping all varieties into spring and winter populations.
Dysferlin expression increases with differentiation, in line with previous data from C2C12 myoblast cells [39].
The expression levels of cDNMT1, cDNMT3A, and cDNMT3B mRNA during early embryonic development, PGC differentiation, germ line development, and sexual maturation were examined at limited time points from stage X to 25 weeks of age by in situ hybridization.
Conversely, DIST displays low H3K9ac and high H3K27me3 signature in GM, while the pattern is reverted upon differentiation, in line with transcriptional activation (highlighted in gray).
Because the number of neuronal differentiations, hiPSC lines and patients studied varies between published reports, we summarize the various methods in Table 1.
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