To examine the relations of a given variable and CIMP-high, we utilized unconditional logistic regression models to calculate odds ratios (ORs) for CIMP-high, according to the status of the given variable, unadjusted and adjusted for age, sex, tumor location, stage, differentiation, LINE-1 methylation level, and status of MSI, KRAS, BRAF, p53 and β-catenin.
This classification revealed previously unrecognized tissue differentiation lines (adipocyte, fibroblastic, smooth-muscle) and was reproduced in paraffin specimens.
To further demonstrate the molecular basis of reclassifying MFH, we examined whether MFH samples overexpressed genes associated with their predicted differentiation lines.
These findings, taken together, suggest that our proposed reclassification is not merely a mathematical model function; rather it is tracking real underlying molecular sarcoma "phenotypes" based on tissue differentiation lines.
There was also a subset of non-overlapping miRNAs (Table 2) which may be more specific to the different sarcoma differentiation lines.
Dysferlin expression increases with differentiation, in line with previous data from C2C12 myoblast cells [39].
The expression levels of cDNMT1, cDNMT3A, and cDNMT3B mRNA during early embryonic development, PGC differentiation, germ line development, and sexual maturation were examined at limited time points from stage X to 25 weeks of age by in situ hybridization.
Conversely, DIST displays low H3K9ac and high H3K27me3 signature in GM, while the pattern is reverted upon differentiation, in line with transcriptional activation (highlighted in gray).
Thousands of H3K4me1-marked enhancers found in undifferentiated ASCs are retained through differentiation, in line with the adipogenic commitment of ASCs [ 55, 56].
Untreated EBs typically showed two pulses of Islet-1 transcription over the first 12 days of EB differentiation (blue line, Fig. 6).
