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Initially, during the first 2 weeks of culture, a lag phase of cellular growth and differentiation is observed in the RWV bioreactors.
In such sublethal conditions where cell death and senescence cannot take place, only alternative differentiation is observed [7].
Alternative differentiation is observed in human keratinocytes lacking p16INK-4a, expressing telomerase and retaining their differentiation capacities (N-hTERT cells) upon sublethal repeated exposures to UVB [7].
The group of genes that has a profound constant up-regulation during differentiation is observed to change also in primary human adipocytes: LXRA (338-fold), PPARG (85-fold), RXRA (2.3-fold), AR (2.3-fold) and EAR2 (1.9-fold).
A similar delay of differentiation is observed also for the other adult neurogenic niche, the subventricular zone, in which neural cells do not attain terminal differentiation in the absence of PC3/Tis21, as the number of 28-day-old BrdU/NeuN-expressing neurons detected at P60 in the olfactory bulb, i.e., in their final migratory destination, decreases significantly.
The memory T-cell differentiation is observed in most cases of acute inflammation.
Similar(48)
In the HMF, reduced differentiation was observed, which is consistent with the observed increase in the production of NPCs/NSCs.
Extended Data Fig. 2 A MG-derived rod differentiation was observed across the whole retinal section.
No overt signs of differentiation were observed in the earlier siRNA approach which led to apoptosis24.
Immunofluorescence (IF) assay for α-SMA expression revealed that WT BALF induced differentiation of fibroblast into myofibroblast, but no differentiation was observed with Ninj1 KO BALF (Fig. 4G).
However, no adipogenic differentiation was observed within the preadipocyte-seeded scaffolds.
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