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Importantly, the ability for oligodendroglial differentiation is maintained after transplantation.
The issue of how genetic differentiation is maintained on such a small spatial is also unclear.
Although postnatally this action maintains the quiescence of muscle satellite cells, the prenatal role is more complex and depends on the environmental context during development, with signalling by myostatin ensuring that a balance between proliferation and differentiation is maintained [19].
The question remains if immunoprivilege of allogeneic WJ-MSC upon differentiation is maintained.
This question is relevant to assess how population genetic differentiation is maintained.
For example, the balance between stemness and differentiation is maintained by the reciprocal regulation miR-145 and the embryonic stem cell genes NANOG, OCT4, SOX2 and KLF4 [77]– [81].
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In this integrated model, the rule for differentiation was maintained between committed cells and corneocytes (as we previously published for our 2D model of keratinocyte colony formation) as in vivo there are gradients of many differentiation related signals.
The medium was changed every 3 days, and the differentiation was maintained for 14 days.
Osteoclastic differentiation was maintained for 12 to 14 days prior to tartrate resistant acid phosphatase (TRAP) staining and scoring of multinucleated OCs, as previously described [ 22].
Those exhibiting homogeneous and robust transgene expression in vitro under both stem cell conditions and conditions employed to promote their differentiation were maintained further.
These are related to MSCs survival and homing capacity after transplantation, the relationship between the host immunity and MSCs, the route of administration (local or systemic) and whether the properties like proliferation, differentiation and trans-differentiation are maintained after in vivo transplantation.
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