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Differentiated PC12 cells were imaged using light microscopy, and ImageJ was used to count the number of differentiated cells (where differentiation is defined as one or more neural extension being longer than the major diameter of the cell body), the number of extensions per cell and the length of extensions.
In our model cell differentiation is defined as the loss of stem cell properties.
In the model, heterogeneous differentiation is defined as the process in which more than one functional (non-naïve) phenotypes can be observed upon uniform treatment of a population of simulated naïve cells (see Methods).
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Sixty-five women had poorly differentiated tumours (G3), while in 33 cases, the histological differentiation was defined as G1-2.
For all cases, pathologic features including tumor differentiation, mucinous features and signet ring cells were examined by a pathologist (S.O .. Poor differentiation was defined as the presence of <50% glandular area.
Mucinous differentiation was defined as fully (>50%), partly (0 50%) or no mucinous differentiation.
Tumour differentiation was defined as good, moderate or poor, as described in the pathology report.
*Spontaneous differentiation was defined as 10 or more myotubes with more than 2 nuclei in a 100 mm culture dish.
Sebaceous differentiations is defined as the presence of multiple cytoplasmic fat vacuoles and the positive staining of tumor cells for EMA and negative staining for CEA [ 10, 11].
MR grading is defined as differentiation between benign and malignant lesions, whereas characterization on MR consists of prediction of the exact histology (tissue-specific diagnosis).
This entire process from epithelial cell to mature fiber cell is defined as lens differentiation.
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