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Our observation that let-7 miRNAs are co-regulated upon BD-TS differentiation is consistent with recent reports that members of the let-7 family can be co-ordinately regulated in a post-transcriptional fashion [39] [42].
Populations examined in this study were selected to represent a high degree of connectivity with the potential for gene flow, and the observed lack of sequence differentiation is consistent with this potential being realized.
Furthermore, the finding that a single dose of BMP-12 was sufficient to induce BM-MSC differentiation is consistent with the activities of other BMP family members like BMP-2, where single, short-term administration is highly effective in cartilage and bone induction [29], [30].
The inhibitory effect of a LIFR antagonist on osteoblast differentiation is consistent with reports that the LIFR ligands LIF and CT-1 stimulate osteoblast differentiation and bone formation [23], [42] and that OSM, acting through LIFR in mice, stimulates bone formation [43].
This functional differentiation is consistent with the anatomical connectivity along the septotemporal axis.
Parallelism of developmental phenotypes between demes belonging to similar thermal groups indicates that the differentiation is consistent with local adaptation.
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Expression of late stage chondrocyte markers such as Col10a1 and molecules involved in matrix turnover, EO, and osteoblast and osteoclast differentiation were consistent with their expected expression patterns [8], [12] [14], [19], [41] [51].
Coalescent simulations were used to test whether patterns of population differentiation are consistent with the single or multiple refuge models.
Our observations that demethylation is a common feature of tissue differentiation are consistent with the recent report that liver development in human is characterized predominantly by demethylation [ 20].
In our previous research, we found that the expression level of adipocyte master regulator C/EBPalpha in osteoblast differentiation was consistent with adipogenic conversion ability.
These findings of greater Wnt transcriptional activity in HSCs undergoing T-lineage differentiation are consistent with the increased levels of β-catenin seen by flow cytometry.
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