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Gene duplications apparently involved functional diversification with consequent differentiation in expression patterns.
The present study found that this gene was strongly up-regulated in resistant mosquitoes and it showed the highest differentiation in expression between resistant and susceptible mosquitoes.
Other studies have shown that stabilizing selection often acts to prevent genetic differentiation in expression patterns among populations and among species [ 57, 58].
Hence it is possible that small differences in absolute transcript levels, which could be an outcome of genetic drift, may have resulted in the strong differentiation in expression of this gene between the strains.
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In contrast, 108 EST clones that can be assigned to 44 different EST clone groups (identical accessions and unique patterns of expression) identified in whole juvenile fish [see Additional file 2] also show significant differentiation in gene expression in white muscle of adult fish of the same dwarf and normal strains in a controlled common environment [ 21].
This difference in the proportion of EST clones that display significant differentiation in gene expression in the two datasets was highly significant (Fisher's Exact test, p < 0.001).
Conversely, CCR7-positive and CCR5-positive cells in the synovial tissue displayed a different distribution, showing an even higher differentiation in their expression in respect to SF.
Some studies have investigated whether hatchery strains and wild fish populations show differentiation in gene expression levels.
Overall there was a clear differentiation in gene expression in the dwarf phenotype in which plant defence was up-regulated whilst plant growth was down-regulated.
The differentiation in marker expression in paired tumour and normal tissues was evaluated by the paired t-test on the transformed values.
Due to the strong differentiation in gene expression between males and females, the ePST were estimated separately by sexes on all transcripts.
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