To evaluate potential targets regulated by BMP signaling in Müller glia, we assayed by qRT-PCR the levels of several transcription factors previously shown to be involved in glial differentiation in explants treated with DM (Nelson et al., 2011).
We next tested the premise of Müller cell-mediated photoreceptor regeneration in explants of rd mice where rod cells degenerate precipitously due to a mutation in the phosphodiesterase gene, using the activation-differentiation paradigm described previously.
Taken together, these data indicate that Wif1 stimulates cardiomyocyte differentiation in PE-explant cultures.
In addition, activin promotes cardiac differentiation of posterior epiblast explants of the chick [4] and induces cardiac tissue in a dose-dependent manner in Xenopus animal cap explants [20], [21].
To elucidate the mechanism by which border cells become specified as neural crest cells, we established an explant assay of neural crest cell differentiation by culturing ectodermal explants of the neural plate border region of stage 4 chick embryos for 20 22 hr, corresponding in time to approximately stage 10 (Fig. 1B).
We examined whether UroA/UAS03 upregulate expression of NQO1 in colon explants of these mice.
Spontaneous myocardial differentiation in chicken PE-explant cultures was first described by Langford et al. [6].
In addition, for one of the factors involved in the Epicardial lock, Wif1, we show with model systems for the first and second heart fields that it enhances cardiomyocyte differentiation in chicken PE explant cultures, increases the Tbx18-positive cardiomyocyte progenitor pool in chicken embryos stimulates cardiomyocyte differentiation in the mouse p19cl6 cell line.
i.e., that show a transient increase in expression early during PE differentiation towards cardiomyocytes in explant cultures.
We therefore explored whether FGF-10, a central inducer of airway outgrowth, induced vascular differentiation in murine fetal lung organ explants.
