Differentiation gene expression analysis of control and Huwe1 knockdown C2C12 cells on differentiation Day 6 by RNA-seq.
DOI: http://dx.doi.org/10.7554/eLife.08536.015 10.7554/eLiFigure36 sourcegure 6—source data 2. Differentiation gene expression analysis of control and Huwe1 knockdown myotubes on differentiation Day 8 by RNA-seq.
DOI: http://dx.doi.org/10.7554/eLife.08536.016 10.7554/eLiFigure36 sourcegure 6—source data 3. Differentiation gene expression analysis of control and Huwe1 knockdown C2C12 cells on differentiation Day 0 by RNA-seq.
As expected from the results of the differentiation assay, gene expression analysis did not show any observable differences in differentiation markers in cells differentiated in the presence of either FGF4 or 8 when compared to controls (Figure 3C&4E).
In fact, the inhibition of miR-20a, miR-9 and miR-92a in the MYCN-non-amplified SK-N-AS cells restored the ability of these cells to respond to RA treatment, as demonstrated by Annexin V assay and differentiation-related gene expression analysis.
We dissected the molecular consequences of matrix release, monolayer, and 3D-alginate culture, growth factor optimised expansion, and re-differentiation protocols by gene expression analysis.
Surprisingly, adult MSC displayed higher differentiation capacities confirmed by gene expression analysis on a selected panel of adipogenesis-related genes.
To study its biological activity on hES cells, we have compared BMP-2, BMP-6 and BMP-2/6 in inducing differentiation of hES cells, quantified by gene expression analysis of specific differentiation markers.
For this purpose, cells were cultured in the presence of BMPs for 0, 3 and 7 days of differentiation and then harvested for gene expression analysis.
Chondrogenic differentiation, assessed by histology, immunohistochemistry, and gene expression analysis, in ATDC5 and articular chondrocyte pellets was evident by the presence of distinct chondrocytes, expressing Sox-9, aggrecan, and type II collagen, in lacunae embedded in a cartilaginous matrix of type II collagen and proteoglycans.
Gene expression analysis during differentiation revealed strongly altered expression profiles in both PKD2 depleted cell lines (#PKD2-2, #PKD2-4; Figure S5A-E).
