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Thus, mASCs undergoing osteogenesis recapitulate the in vivo osteogenic differentiation expression pattern of FGF ligands and receptors of calvarial mesenchymal cells during their own osteogenic differentiation.
GBM malignancy is driven in part by a population of GBM stem-like cells (GSCs) that exhibit indefinite self-renewal capacity, multipotent differentiation, expression of neural stem cell markers, and resistance to conventional treatments.
To identify changes in gene expression that underlie this disrupted differentiation, expression microarray analysis was performed.
After induction of differentiation, expression of miR-489 becomes almost undetectable, decreasing 10-fold (p-value<0.1) (Table S1).
These results suggest that Ker-CT-Ras keratinocytes maintain their differentiation expression profile irrespective of their randomized location, but that subsequent modifications alter the cellular expression profile.
In order to demonstrate that knockdown of SMS1 and SMS2 by siRNA did not affect differentiation, expression of the neutrophil cell-surface marker CD11b was determined.
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In fact, a study has reported that during monocyte-macrophage differentiation, expressions of miR-15a and miR-16 were decreased with higher expression of the IKKα [ 30].
Expression of CD18 increased with differentiation, while expression of CD36 decreased (data not shown).
However, during lymphoid differentiation, ALDH expression apparently diminishes before the loss of CD34 expression.
Ninety-one days after transfection (2 weeks after the stimulus for each differentiation), osteocalcin expression was induced, but neither FABP nor aggrecan were expressed (Figure 5b).
Detailed time-course analysis of epidermal differentiation marker expression in BCC.
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