Sentence examples for differentiation day from inspiring English sources
The neuroblastoma cells begin to get in contact with the support already on the early days of differentiation (day 5).
After removal of the culture medium undifferentiated cells (day 0) and cells after 28 days of differentiation (day 28) were washed twice with ice cold PBS (pH 7.4).
To gain global insights into gene expression alterations upon ZEB1 KD, we performed replicate RNA-seq experiments in control and ZEB1 KD cells prior to differentiation (day 0) and 2 days after the onset of differentiation ('Materials and methods').
Mouse ES or human ES differentiated cells at terminal differentiation day were digested into single cells with Accutase.
Two days post-confluence (differentiation day 0) medium containing 0.5mM isobutylmethylxanthine (IBMX), 1μM dexamethasone and 5μg/ml insulin (MDI cocktail) and 10% fetal calf serum (FCS) was added.
Two days after the initiation of differentiation (Day 2), the culture medium was replaced with DMEM supplemented with only 10 μg/ml insulin and 10% FBS.
As we expected, expression of the pluripotency markers Oct4 (Pou5f1) and Nanog declined gradually as the cells differentiated, down to their lowest level of expression on differentiation day 9. AHR activation, knockdown, and inhibition all block cardiomyocyte lineage differentiation.
For bone induction, the most commonly utilized BMPs are BMP2 and 7. BMP2 acts upstream inducing global cellular mobilization (day 1 to 3), whereas BMP7 acts on bone differentiation (day 2 to 5) [[2]].
Myotubes were treated as indicated on differentiation day 6.
We evaluated the levels of both of these enzymes in transduced cells during the proliferative phase of differentiation (day 14), the nonmineralizing hypertrophic phase of differentiation (day 28), and the mineralization phase of differentiation (day 35).
Comparative similarity in mRNA pools was observed to rapidly diverge when the laboratory strains were compared to populations prior to and following the initiation of bradyzoite differentiation (Day-15 and pH-shifted populations).
