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Immunostaining for cardiomyocyte, smooth muscle and endothelial markers after 14 days of co-culture demonstrated that the majority of the CPCs differentiated into cardiomyocytes (Fig. 2c) with limited smooth muscle or endothelial cell differentiation (data not shown).
The experiment was prolonged to 10 days since we did not observe a significant increase in GFAP expression in control cells after 7 days of differentiation (data not shown).
Our initial experiments were designed to investigate whether BPA could replace any of these components, and it was found that BPA could only replace the DEX-mediated effects on differentiation (data not shown and Figure 1).
When applied to mouse embryonic stem cell differentiation data, SPD uncovered a landscape of ESC differentiation into various lineages and genes that represent both generic and lineage specific processes.
When applied to B-cell dataerentiation data, SPD recovered the correct order of stages of normal B-cell differentiation and the linkage between preB-ALL tumor cells with their cell origin preB.
Differentiation data showed that the cells were significantly affected by the topographical cues of the underlying graphene surfaces.
Indeed, microarray studies revealed that similar to cancer cells and preimplantation embryonic cells, several SAC proteins such as MAD2 and BUB1 are highly expressed in undifferentiated hESC and are down-regulated upon differentiation (data obtained from www.amazonia.transcriptome.eu).eu
Spry4 expression was relatively unchanged during ES cell differentiation (data not shown).
Other DAG species did not change significantly during differentiation (data not shown).
Additional supplementation with DKK1 and VEGFA165 [13] did not further enhance differentiation (data not shown).
CD56+ cells gave rise to myotubes from the second day after initiating differentiation (data not shown).
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