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As long as passages are performed according to strict cell culture guidelines (Additional Methods - see Additional file 1), appropriate Matrigel™ lots should reproducibly trigger basoapical polarity in a differentiation capable cell line, as shown by a defined range of basoapical markers.
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In order to keep a high percentage of differentiation-capable cells, a defined concentration of cells is used for plating for monolayer and 3D cultures and cells are kept in culture for 10 passages maximum, with passage 60 as the latest possible passage used for 3D culture assays.
In vitro and in vivo studies have shown that human bone morphogenetic protein-2 (hBMP-2) is an important developmental growth and differentiation factor, capable of inducing ectopic bone formation in vivo.
These limitations highlight the need for a reproducible, fully optimized and universally applicable differentiation system capable of overcoming the interline variability that commonly exists amongst human pluripotent stem cells (hPSC).
It is also a major part of a differentiation cocktail capable of inducing Schwann cell differentiation from neural stem cells [15], bone-marrow stromal cells [16], adipose tissue-derived stem cells [17] or human umbilical cord-derived mesenchymal stromal cells [18].
The so-called skin-derived progenitors are another kind of skin cells with high differentiation potential capable of hepatogenic differentiation.
To date, differentiation protocols capable of generating photoreceptor precursor cells from both ESCs and iPSCs have been developed (13– 23).
Mesenchymal stem cells (MSCs) are pluripotent progenitors with multilineage differentiation potential, capable of undergoing osteogenesis, adipogenesis and chondrogenesis.
A protocol with the specified aim of creating a differentiation process capable of creating dopaminergic neurons for transplantation in T-flasks has been developed [ 89].
However, it can be imagined as well that highly aggressive tumor cells that are naturally deficient in XOR expression[134 136] may be both poorly set up to promote differentiation but still capable of responding to exogenous UA by increased aggressiveness.
These data strongly support that our differentiation conditions are capable of directing the generation of NHEM-like epidermal melanocytes from iPS cells (Fig. 3U).
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