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The spheres were subsequently used for blood cell differentiation according to the established protocol20.
When the value-added factor is high, many opportunities exist for differentiation according to product characteristics and cost structures.
We show that communication between cell-mimics allows distribution of tasks, quorum sensing, and cellular differentiation according to local environment.
The differentiation and maturation process was completed at day 19 after initiation of differentiation, according to our gene expression profile results.
We aimed to emulate cellular differentiation according to local environment by combining the artificial quorum sensing network with a constitutively expressed tetR-mCherry reporter that turns on irrespective of cell-mimic density (Fig. 4d).
We observed that alkaline phosphatase (ALP) was largely expressed in the earlier stage of osteoblastic differentiation according to histochemical staining and RT-PCR analysis, whereas osteocalcin was dominantly expressed and secreted into the medium at the later stage.
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Differentiations according to criteria of cognitive psychology (types of knowledge) hardly exist in the non-academic field: Until now, only Pittich (2013) claims to have separated conceptual knowledge from other types of knowledgee.
Assays of in vitro differentiation to osteocytes, chondrocytes, and adipocytes were performed using StemPro Osteogenesis Differentiation Kit, StemPro Chondrogenesis Differentiation Kit, and a StemPro Adipogenesis Differentiation Kit according to the manufacturer's protocol.
Human white preadipocytes were differentiated into adipocytes in culture with a commercially available preadipocyte differentiation medium according to the manufacturer's instructions (PromoCell).
Alternatively to such two-stage process, Murphy and Brownell [5] proposed a differentiation theory according to which all category representations would be activated in parallel.
Cells were maintained and differentiation induced according to the manufacturer's protocol.
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