To differentiate the expression of the selected miRNAs and to categorize them according to their expression patterns, a heatmap chart was drawn by transforming the normalized data to a log 2 scale for visualization purpose.
To be able to differentiate the expression of each miRNA and categorize them according to their expression pattern, a heatmap chart was drawn by transforming the normalized data to log 2 scales for visualization purpose.
Strain-resolved proteomics has been used to differentiate the expression of co-occurring protein variants within a single sample of activated sludge cultivated for EBPR in the United Kingdom and dominated by A. phosphatis (Wilmes et al., 2008a; Fig. 3b).
To be able to differentiate the expression of each miRNA and categorise them according to their expression pattern, a heatmap chart was drawn and hierarchical clustering with Euclidean distance and complete linkage algorithms was performed using the MeV online software (http://www.tm4.org/mev.html).
Since we wanted to optimize chances of differentiating the expression signals from ohnologs of the recent WGD, 50 mer oligonucleotide probes were chosen.
The idea is that when cells differentiate, the expression levels of one TATA binding factor decrease whereas those of another increase, effectively switching the gene-regulatory control from one factor to the other.
Although our current data confirm the increased miR-146a levels in synovial and peripheral blood CD4+T cells of RA patients, we were not able to differentiate the expression pattern of miR-146a levels in various CD4+T cell subpopulations due to the limited numbers of synovial and peripheral blood CD4+T cells.
In the sik1flp/flp differentiating EBs, the expression of these genes was impaired in this time window; in line with the morphological analysis, the expression of the cardiac structural genes (α-MyHC, Mlc2V, αCach and cTnI) was impaired from day 6 to day 8, whereas it increased from day 9 onward to reach levels comparable to wt cells.
