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The modes of distribution are provided by differentiating the density of new Burr distribution in 1.6 with respect to x: begin{aligned} f^(x mu,sigma,p =3left( frac{x-mu }{sigma }right) ^3left( 1-pe^{-left( frac{x-mu }{sigma }right) ^3}right) -2left( 1+e^{-left( frac{x-mu }{sigma }right) ^3}right).
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These findings are consistent with our hypothesis that the density of differentiating sites complicates the mapping of reads and leads to biased measures of relative ASE.
To determine whether this bias was influenced by the density of differentiating sites, we calculated the maximum number of sites that differed between the two alleles among all possible 36-base reads overlapping each differentiating site.
After each of these alignments, we quantified ASE, measured the density of differentiating sites, and determined the mappability to each genome using the same strategies described above for the simulated data.
The major consequence of excluding sites based on the density of differentiating sites, mappability, and/or indels is that fewer allele-specific reads will be successfully mapped for each exon and for each gene.
Using both simulated and real sequencing data, we found that sites affected by the systematic bias toward the reference allele could be identified and excluded prior to estimating ASE based on the density of differentiating sites.
In particular, the density of polymorphism differentiating the two species was 31.35 SNPs/InDels for loci related to saprobic growth, 21.25 SNPs/InDels per kilobase for loci associated with sporulation, and 20.43 SNPs/InDels per kilobase for loci associated with pathogenicity.
The density of microtubules in differentiating cells precludes a meaningful analysis of bundling with light microscopy methods.
Similarly, differentiation efficiency did not correlate with the density of either total cells or desmin+ cells; satellite cells are capable of differentiating (expressing MyHC) independently of cell fusion.
The quantity comes directly from differentiating the equation of state.
The density of blood vessels generated by DPSCs differentiated into vascular endothelial cells was analyzed by immunohistochemistry for EGFP.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com