The heart of this method lies in the use it makes of definitions, conceived of as differentiating marks of the thing defined.
The meiotic drive hypothesis was supported by these mapping data because there is a positive correlation between the number of inversions differentiating the marked and wild chromosomes and the amount of deviation from Mendelian expectations (Figure S1).
The classical view is that to restore the germline to the totipotent state of the early preimplantation embryo, differentiating epigenetic marks are removed by two phases of epigenetic reprogramming: at the PGC stage until just after their entry in the incipient gonad and after fusion of sperm and oocyte in the zygote and during the first cleavage divisions (Morgan et al., 2005; Feng et al., 2010).
Make sure you can differentiate your marks from those that other cavers may have left.
In this case, either allele could be methylated, or there may be a preference for acquiring methylation on one parental allele due to other epigenetic marks differentiating the two parental chromosomes.
Hence, undifferentiated spermatogonia are Smc6-negative and expression of Smc6 marks differentiating spermatogonia.
To verify that the Smc6 expression marks differentiating spermatogonia, we conducted immunohistochemistry for both Smc6 and LIN28 on consecutive sections of mouse testes.
Haplotype comparison is useful for identifying larger genomic tracts differentiating accessions or marking historical introgressions as in rice [ 39] and maize [ 34], or for assessing patterns of linkage disequilibrium as in soybean [ 36].
We assayed nuclear Prospero, which marks differentiating GMCs and young neurons in the larval CNS (Carney et al., 2013), Embryonic lethal and abnormal vision (Elav), which marks all neurons (Robinow and White, 1988); and Reversed polarity (Repo), which marks all non-midline glia (Xiong et al., 1994).
Cash1 expression forces cells out of a proliferative state and toward differentiation, marking a transient stage of the cell as it differentiates into a neuron [26].
Finding that Lbe marks specifically differentiating leg myoblasts suggested an instructive role in leg myogenesis.
