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Further, the orthologous murine signature correctly orders a time course of differentiating embryonic mouse stem cells.
The biological activities of YY1 in CPCs appear to be cell autonomous, based coculture assays in differentiating embryonic stem cells.
Magli, A. et al. Pax3 and Tbx5 specify whether PDGFRalpha+ cells assume skeletal or cardiac muscle fate in differentiating embryonic stem cells.
There is an intense interest in differentiating embryonic stem cells to engineer biological pacemakers as an alternative to electronic pacemakers for patients with cardiac pacemaker function deficiency.
Here, we show that TBX3 is dynamically expressed during specification of the mesendoderm lineages in differentiating embryonic stem cells (ESCs) in vitro and in developing mouse and Xenopus embryos in vivo.
By comparing our results with those recently reported on mRNA decay in pluripotent and differentiating Embryonic Stem (ES) cells [10], we found that GREs are significant in different cell types, whereas some AREs show cell-specific activities.
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In stark contrast, newly differentiated embryonic stem cells exclusively expressed full-length MUC1 but not the cleaved form, MUC1*.
Specific transcription factors need to be expressed to differentiate embryonic cells into these specific tissues, and transcription factor activity was the most overrepresented molecular function.
To get a comprehensive view, we also incorporated 24 differentiated embryonic stem cell samples in addition to the 31 wild-type embryonic stem cell samples and 16 normal adult human tissues.
Kobayakawa, S., Miike, K., Nakao, M. & Abe, K. Dynamic changes in the epigenomic state and nuclear organization of differentiating mouse embryonic stem cells.
Available methods for differentiating human embryonic stem cells (ESCs) and induced pluripotent cells (iPSCs) into neurons are often cumbersome, slow, and variable.
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