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Another line, selected to express even lower levels of the activator, behaves like a typical ESC, subject to differentiation upon LIF removal, despite the very low level of expression of the activator fusion, and this line differentiates upon expression of additional Oct4.
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Although RA is required for proper maintenance of epithelial tissues, the binary nature of RA-induced differentiation, in which cells commit to differentiate upon a defined time interval, may not be the ideal setting for the characterization of long-paced oscillations of NR activity.
We chose SK-N-BE(2) cells for the differentiation experiments because they are known to differentiate upon downregulation of N-Myc.
Overall, hPSC-LESCs retained their capacity to self-renew and proliferate, but were also able to terminally differentiate upon stimulation, as suggested by protein expression of cytokeratins 3 and 12.
Control EpiSCs and human ESCs similarly differentiated upon ALK-1 inhibition (data not shown).
Creating stably transfected lines from hESC is difficult since the cells tend to differentiate upon long-term selection.
Embryonic liver samples exhibited a gene expression profile that differentiated upon progression in the developmental process, and revealed multiple regulated genes.
These progenitors differentiate upon the concerted and combinatorial action of four highly conserved signal transduction pathways triggered by Wingless (Wg)/Wnt, Decapentaplegic (Dpp /TGF-β, Receptor Tyrosine Kinase (RTK)-Ras-MAPK anDpp /TGF-β) [10].
Pluripotent progenitors are defined by their ability to maintain and differentiate upon exposure to specific stimuli.
This etiolated seedling pathway profile represented a heterotrophic tissue poised to differentiate upon an environmental cue.
In comparison to control cells, which differentiated upon Oct4 transfection, Nanog/T cells formed morphologically undifferentiated colonies.
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CEO of Professional Science Editing for Scientists @ prosciediting.com