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It is important to highlight that exogenous expression of ΔNp63 in ES-derived ectodermal cells efficiently differentiates them into keratinocytes and that in zebrafish, knockdown of ΔNp63 leads to a block in epidermal proliferation and development [34], [52].
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"We've done extensive tests in Petri dishes in vitro by differentiating them into cardiac cells, into muscle cells, into neurons and then measuring their ability to produce energy.
However, scientists are now able to convert patient fibroblasts into iPSCs and differentiate them into disease-relevant cell types.
It was the first study which recognized two kinds of passenger-delay costs differentiating them into "hard -monetary measurable and "soft"- as hard -monetaryy measurable ones.
Accordingly, efforts have been undertaken in order to efficiently reprogram somatic cells to pluripotency, and then differentiate them into pure cultures of specific cell lineages.
For the computation of the profit of a possible degradation, "revenue weights" are computed and assigned for each one of the ten "modes", thereby differentiating them into different service classes.
Culturing human adipose-derived mesenchymal stem cells (ADMSCs) on top of HGF/Col spots (HGF co-printed with collagen I to create arrays of protein spots on glass) for 2 weeks can differentiate them into HLCs (Ghaedi et al., 2011).
More important [is] to then differentiate them into a cell type that is relevant for a disease... and then try to understand the disease in a dish," says Martin Graf, head of the stem cell platform at Hoffmann-La Hoffmann-La Rochewinzerland and one of the leaders of the StemBasel project.
Cells were grown for several weeks in DMEM containing 2% B27, 1% PSF to differentiate them into neurons.
To confirm the neurite phenotype observed in NSC-34 cells, we transfected the constructs into the rat forebrain neural precursors and then differentiated them into neurons.
The ability to expand human embryonic stem cells (hESCs) unlimitedly in culture and to differentiate them into specific somatic cell types [1] make them a useful tool in the development of hESC-based automated screening platforms for drug discovery.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com