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They generally tend to occur in the 5th and 6th decades of life and can be classified into four subtypes: well differentiated with and without dedifferentiated components, myxoid and round cell liposarcoma and pleomorphic liposarcoma [1].
(+++ = high expression; + = normal expression; +/- = partial expression, - = not expressed; N.D. = not determined) In order to examine the molecular basis of the effects of RA on myogenesis, total RNA was harvested from a time course of cells differentiated with and without RA and subjected to northern blot analysis.
We determined the phenotype of DC differentiated with and without recombinant VEGF or RCC supernatants.
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Previous preliminary experiments confirmed that on days 14 and 21 differentiated with cortisone and days 8 and 16 differentiated with cortisol, cells had reached comparable differentiation states (assessed lipid accumulation and ΔCT values of differentiation markers) due to the greater differentiation capacity of the active steroid.
For all other experiments, gene expression was analyzed on days 7, 14, and 21 in cells differentiated with cortisone and on days 8 and 16 for cells differentiated with cortisol.
Interestingly, there was no difference in platelet production between primary MKs that were overexpressing Livin-RING compared with empty vector and CD34+ cells differentiated with TPO and SCF factor alone.
Adenocarcinomas were poorly differentiated, with cellular and nuclear pleomorphism, high mitotic rate, and evidence of invasion.
ASCs were also differentiated with ATRA and transfected with anti-miRNA let-7e for knockdown at day 4 and collected at day 6.
PC12 cells were differentiated with NGF and all experiments were conducted at ∼20% confluence.
From 13 dpi on, the collected nodules were differentiated with zonation and with a pink colour indicative of nitrogen fixation.
PC12 neuronal cells were differentiated with NGF and exposed in vitro to sub-cytotoxic concentrations of iron and/or alpha-methyltyrosine (AMT), an inhibitor of tyrosine hydroxylase (TH), the rate limiting enzyme in the biosynthesis of dopamine.
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