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Relative mRNA expression of core clock genes Bmal1, Clock, Cry1, Cry2, Per1, Per2, and Per3 in spontaneously differentiated wild type and Clock-/ mESCs after serum shock induced biological rhythms.
(A and B) Immunofluorescence staining of the endoderm marker FOXA2 and the ectoderm marker SOX1 in spontaneously differentiated wild type and Clock-/ mESCs on day 9. Clock-/ mESCs exhibited relatively stronger expression of both FOXA2 and SOX1.
Furthermore, levels of TAG from differentiated Fsp27−/− MEFs were significantly lower than that of differentiated wild type MEFs (Fig. 6B).
In addition, protein levels for mitochondrial proteins for oxidative phosphorylation such as cytochrome-C and COXIV were also similar in differentiated wild type and Fsp27−/− MEFs.
Ucp1 protein was also induced in the presence of T3 in differentiated wild type MEFs but its levels were increased by approximately two fold in differentiated Fsp27−/− MEFs (P<0.05).
In the absence of T3, protein levels for PGC1α were similar for differentiated wild type and Fsp27−/− MEFs (Fig. 6D), which is in drastic contrast to the phenotypic changes seen between the wildtype and mutant adipocytes derive from the mice.
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Testicles transplanted with Pten−/− differentiated cells were five times larger than unmanipulated testicles or testicles transplanted with differentiated wild-type cells of equal cell number (Fig. 2A).
The authors observed that, compared to differentiated wild-type myotubes, FSHD myotubes were thinner, less branched, more disorganized and were comprised of fewer myoblasts as measured by total number of nuclei per myotube.
As expected, TERT transcription was undetectable in differentiated wild-type fibroblasts.
Differentiated wild-type podocytes (5 × 10) were seeded overnight in six-well plates.
When alkaline stressed, the tachyzoite-like Δ gcn5A mutants (GSE22100) and their differentiated wild-type counterparts [ 12] were enriched for only one gene set derived from KEGG terms.
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