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rMAPC were differentiated using the same protocol, except that the differentiation was done without FCS and the starting cell density was 50,000 cells/cm2 (as described in [38]).
Single-analyte spectra were normalized and differentiated using the same settings for comparison.
P2rx7−/− and Cd39−/− BMMCs were in vitro differentiated using the same protocol but starting from bone marrow cells of 6 to 8 week old P2rx7−/− and Cd39−/− (both C57Bl/6) mice.
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Fall injury mechanisms were differentiated using the following distinctions: falls on the same level versus multilevel falls or falls from stair/steps, falls from household furniture versus falls from stairs/steps or playground, and low versus high height falls.
It is also important to note that two different arrangements of χ(2) scatterers within the focal volume, although generating the same SHG signal, can be differentiated using the radiation pattern.
Furthermore, a AdMyoD was in vitro transduced in differentiated myotubes using the same conditions to the previous AdT transfections.
SHIP1+/+ and −/− BMMCs as well as Lyn+/+ and Lyn−/− BMMCs were differentiated in vitro using the same protocol but starting from bone marrow cells of 6 to 8 week old SHIP1+/+ and −/− littermates (129/Sv × C57BL/6).
The inducible shRNA hESC lines were then differentiated into endothelial lineage cells using the same protocol with or without doxycycline treatment from day 4.
To produce sufficient cells for CPT1B protein quantification, C2C12 cells were instead grown and differentiated in collagen I-coated T75 flasks (Millipore, USA), using the same medium-change procedure and nitrate/inhibitor concentrations as above.
In separate experiments cells were differentiated in the presence of 10 µM testosterone (T) (Mouriec et al., 2009) using the same protocol.
Nevertheless, striatum clearly differentiated from neocortex in the amount and chromosomal locations of sex-biased genes, and these two tissues were analysed using the same platform.
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