Such lipomas cannot be distinguished from well-differentiated liposarcoma with imaging alone, and biopsy is mandatory to exclude well-differentiated liposarcoma and the possibility of dedifferentiation [17].
Sorted lineage negative BM cells (lin−; CD3e−, B220−, CD19− CD11b−, Gr-1−, TER-119−, NK1.1− and CD127−) were used to exclude any contribution from differentiated cells and lymphoid progenitors (Fig 1C).
To exclude non-specific binding, we used the differentiated ES (dES) cells and primary mouse embryonic fibroblast (PMEF) cells, a feeder layer for the maintenance pluripotency of embryonic stem cells, as a control.
To distinguish between these possibilities, we analysed microtubule motility in differentiated, shRNA-treated cells, again selecting elongated, mono-nucleated cells to exclude effects due to morphology alone.
To exclude mixed, non-SQCLC tumor compositions, the highest differentiated sections in the tumors were selected for IHC analysis and DNA extraction.
Since 1322T tumours contain high frequencies of Paneth cells, which appear to be terminally differentiated and hence not to be progenitors of tumour growth, we used laser capture microdissection to exclude this lineage from the analysis.
This was done in order to exclude the possibility of a misinterpretation and misclassification of dedifferentiated salivary gland carcinomas as poorly differentiated SCC's.
They were designed to exclude.
To exclude the possibility that our triploid XXY ES cells lost or gained X chromosomes over the differentiation process, we performed DNA-FISH with an X chromosome specific BAC probe on cells differentiated for seven days.
