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By tuning differentiation time and medium composition, NPCs were differentiated to a mixture consisting predominantly of GFAP+/nestin+ astrocytes and nestin+/GFAP-/βIII tubulin- progenitors that could effectively induce TEER compared to mixtures containing βIII tubulin+ neurons as the major population.
The tail bud is formed from parts that have already been differentiated to a certain extent; prolongations of the neural tube and of the notochord are involved, and endoderm extends into the tail rudiment as the postanal gut, which, however, soon degenerates.
Adipose-derived stem cells were isolated from rats and differentiated to a Schwann cell-like phenotype in vitro.
Here, we proposed it for stem cell capsulation for future works that stem cells should be differentiated to a specific cell line such as bone or insulin-producing cells, inside the capsules and then transplanted.
MSCs cultured in NFSs in the presence of TGF-β1 differentiated to a chondrocytic phenotype, as evidenced by chondrocyte-specific gene expression and synthesis of cartilage-associated extracellular matrix (ECM) proteins.
It is also important to consider whether a mutation that does not affect the phenotype and behavior of hPSCs may have harmful effects once the cells are differentiated to a particular lineage of interest and/or transplanted into a different niche in vivo.
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The LM was robustly differentiated to an epicardial lineage by activation of WNT, BMP and retinoic acid signalling pathways.
Dysregulation of the miR-143 and -145 genes is suggested to underlie the inappropriate SMC plasticity observed with vascular disease, by promoting a phenotypic switch from a differentiated to an undifferentiated, proliferative and migratory state.
These polynomials are then differentiated to compute a high order accurate numerical solution.
However, the cells must be differentiated to assume a functional neutrophil-like phenotype and to respond to typical agonists, such as f-Met-Leu-Phe.
THP-1 can easily be transfected or virally transduced and do not need to be differentiated to reach a chemotaxis-competent phenotype.
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