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We used real-time PCR and fluorescence spectrophotometer to analyze the variation of aP2 gene expression and hrGFP intensity among different differentiated time points.
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The medium was replaced every other day and the resulting EBs were differentiated for different time points and collected for subsequent analysis.
Treatment with p55-gag significantly reduces levels of RUNX-2 activity relative to differentiated control at all time points.
Depending on the concentrations of the factors and analysis time points, differentiated hiPSC should present these markers [ 65 ].
The Mann–Whitney U-test was used for the statistical analysis of differences between undifferentiated control cells and differentiated cells at each time points (*p).
In addition, cytokine release analysis showed that LL-37-differentiated monocytes released lower levels of IL-1β, IL-6, MCP-1, but higher levels of IL-1ra than osteoclasts after differentiation for all time points analysed (Figure 2K).
As expression of the neuroactive ligand-receptor interactions pathway is demonstrated to correlate with synaptic maturity [ 29], this data is consistent both with the telencephalon being less differentiated at this time point and more importantly implicates a critical role for miRNA in this process.
The concentration of BACE substrate used was that which best differentiated serial CSF dilutions over different time points.
The share of differentiated cells was measured at the time points of 24, 48 and 72 hours.
Reductions in RUNX-2 activity relative to differentiated control was also observed at all time points, and the secretion of BMP-2 is also reduced relative to differentiated control at 9 and 15 days.
We used the Multiparameter Cytotoxicity 1 HCS Reagent kit (Cellomics) for the assessment of overall culture viability using multiple cell parameters in differentiated human and mouse neurons at different time points.
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