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The UPL probes clearly differentiated the same two major Genogroups that those recognized by sequencing analysis.
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It is fundamental to epigenetics that as cell types differentiate the same DNA sequence may display multiple epitypes and some epitypes may be more or less stable than others.
To determine whether the hybrid cells resemble the hFL cells, or on the contrary retained the transcription states of both types of fused cells, we performed a genome-wide transcriptional profiling (Fig. 4) using Affymetrix oligonucleotide arrays on HEF cells, hFL cells differentiated along the erythroid lineage (dhFL), and hybrid cells differentiated in the same conditions (dHybrids).
The neural progenitors and neurons were paired if they were differentiated from the same iPSC clone and plated for differentiation in one experiment.
With interview performed by trained personnel, migraine and TTH can be differentiated in the same person, but with questionnaires, it is usually wise to let the patient answer the questionnaire based on the altogether most bothersome headache [4].
No neurons were detected in P19[Control] cells differentiated under the same conditions (Fig. 2D).
To verify whether the increased mRNA levels were accompanied by alterations in protein levels, GluD1 protein was quantitated in extracts from mature neurons differentiated with the same protocol.
LC differentiation from dox30+ to dox90+ cells was carried out in the absence of doxorubicin, and the non-exposed hTERT-MUTZ3 control cells were always differentiated from the same passage number as the drug-exposed dox30+ and dox90+ cells.
The close resemblance between the members of cluster T (Arn1/Arn2 homologs) and cluster S (Gex1/Gex2 homologs) suggests that these two functionally distinct groups of 14-spanner MFS transporters have been differentiated from the same ancestral gene.
P2rx7−/− and Cd39−/− BMMCs were in vitro differentiated using the same protocol but starting from bone marrow cells of 6 to 8 week old P2rx7−/− and Cd39−/− (both C57Bl/6) mice.
The incidence of ICC by age distribution in patients with either viral hepatitis B or C was nearly identical to that of HCC in patients with viral hepatitis B or C. The ratio of the incidence of HCC to ICC to CHC might reflect the ratio of the numbers of hepatocytes and cholangiocytes differentiated from the same hepatic progenitor cells.
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