This appeared as differentiated temperature-related reaction norms for these traits.
Then the cells were stained in 0.5% Oil Red O-isopropanol working solution for 15 minutes at room temperature, differentiated in 60% isopropanol, and rinsed in tap water.
Samples were stained for cartilage with 15 mg/ml Alcian blue 8 GX in 76% ethanol and 20% glacial acetic acid for 2 weeks at room temperature, differentiated in 95% ethanol and cleared in 1% potassium hydroxide (KOH) until bones were visible.
The growth behavior of Ag nanostructures is remarkably differentiated at higher annealing temperature (750 °C) due to the sublimation and temperature-dependent characteristic of dewetting process.
These variables were therefore included as covariates so as to study whether demes and/or temperature groups differentiated in covariate-adjusted trait values - indicating genetic differentiation under the prevailing common-garden conditions.
Slides were incubated in Oil Red O (ORO; Sigma, Ireland) solution (0.5% ORO in 60% ethanol) for 1 h at room temperature and differentiated by rinsing in 60% ethanol and 4 washes in water.
The blends presented three very well differentiated types of behaviour to temperature depending on the erucamide content.
These findings corroborate, spatially differentiated, the hypothesis that air temperature is a significant driver for phenological development.
For simplification, many studies omit the use of different varieties that are differentiated only by their required temperature sums to reach their respective development stages.
Temperature is spatially differentiated using a vertical lapse (λ t ) rate, which is a calibration parameter.
