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When myoblast was treated with 1 α,25(OH)2D3 throughout myogenesis including proliferative, differentiating, and differentiated stages, myotube formation was delayed by day 10 concomitantly with downregulation of Myf5 and myogenin [ 32].
These results demonstrate that there are major quantitative changes in leptin responsiveness between obese and lean adipocytes during differentiating stages, but not differentiated stages, of hAT in vitro.
However, within the B and T/NK categories two major groups are recognised precursor neoplasms, corresponding to the early stages of differentiation, and mature or peripheral neoplasms, corresponding to more differentiated stages.
A significantly higher T-cell depletion (revealed by mixed lymphocyte reaction, [MLR]) was found for AMSCs (vs. BM-MSCs) at both non- and differentiated stages.
Previous work has established that gametocytes, sexually differentiated stages that are taken up by the mosquito vector, control expression of genes required for mosquito colonization by translational repression.
By using a variety of differentiated stages of NPC cell lines, the UBE2C expression profiles were further analyzed.
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Adenovirus treatment was performed at three different stages: (1) proliferating myocytes at ∼50% confluency (proliferation stage); (2) proliferating myocytes at ∼90% confluency before induction of differentiation (fusion stage); and (3) differentiating cells 3 days after the induction of differentiation (differentiated stage).
By contrast, Okuno et al. [ 27] investigated the effects of 1 α,25(OH)2D3 (1 100 nM) on differentiating and differentiated stage of C2C12 myoblasts.
By utilizing a Cancer Array, containing 440 oncogenes and tumor suppressors to profile mRNA expression, we identified a larger number of differentially expressed genes in poorly differentiated stage II colorectal adenocarcinoma tissues, compared to their matched normal tissues.
The pathology report indicated Tumor 1, Tumor 2, and Tumor 3 were moderately differentiated stage three tumor with no metastasis, high grade poorly differentiated stage three tumor with metastasis to one lymph node, and low grade well differentiated stage three tumor with no metastasis, respectively.
Cd44 at the protein level also decreased with differentiation, although occasional cells remained positive also in the differentiated stage.
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