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Baudrillard erases autonomous and differentiated spheres of the economy, polity, society, and culture posited by classical social theory in favor of an implosive theory that also crosses disciplinary boundaries, thus mixing philosophy and social theory into a broader form of social diagnosis and philosophical play.
Differentiated spheres were attached onto coverslips (Fisherbrand/Fisher Scientific, Pittsburgh, PA, USA) coated with Matrigel for immunofluorescence staining.
Figure 3(A) shows the quantitative analysis of the percentage of PDX-1, Isl-1 and CK-19 positivity in spheres, differentiated spheres (cells in DM for 11 days) and adherent cells.
Based on the enrichment of low-differentiation cholangiocarcinoma cells by sphere formation and drug resistance, we considered that the proportion of cholangiocarcinoma stem-like cells had been increased, and we next validated the miR-200b/c expression levels of the parental and differentiated spheres and drug-resistant cells.
As individuals, she writes, "we are many-sided, if not protean, personalities," and we each inhabit many "differentiated spheres with their own identifiable norms and institutions".
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Similarly, when the impact of cisplatin on cell viability was examined, respectively, in the parental A2780 cells, the differentiated sphere cells, and the sphere cells), significant difference (P < 0.001) was detected between the sphere cells and the A2780 cells/the differentiated sphere cells.
For viability, migration, and differentiation analyses, neurospheres were preincubated for 1 week with PBDEs (0.1, 1, or 10 μM) in proliferation medium; afterward, differentiation was initiated and spheres differentiated with the same concentrations of PBDEs in differentiation medium for 48 hr (migration measurements) or 7 days (differentiation analyses).
First, to elucidate whether the PC-3 sphere cells turned into differentiated cells when sphere cells were digested into single cells for re-adherent culture (10% FBS-RPMI-1640 medium), we compared the expression levels of cancer stem cells markers, such as Oct4, Sox2 and Nanog by qRT-PCR.
The results showed that compared with a static flask culture, MSCs encapsulated in volvox spheres differentiated into hepatocyte-like cells with a 2-fold increase in albumin (ALB) expression and a 2.5-fold increase in cytokeratin 18 expression in a dynamic bioreactor.
The lines expressed neural lineage markers nestin, Sox1, and Pax6 when differentiated either as spheres or in adherent culture for seven days, and two weeks later vimentin, BLBP, GLAST, and GFAP as reported earlier [11].
As tumour spheres differentiated, cells migrated out of the adherent spherical colony to form a flat monolayer with typical epitheloid morphology.
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