We selected 12 outpatient departments in three mental health institutes in The Netherlands, striving for a differentiated sample of locations, according to degree of treatment specialization, nature and severity of psychopathology, and geographical dispersion.
Osteogenic differentiation at day 21 and day 35 was quantified by the index of osteogenic differentiation (IOD IODOD = optical density of differentiated samples optical density of controls Chondrogenic differentiation of P3 MSCs was performed in a 3D-pellet culture system.
The inset in Figure 2 panel J illustrates some of the few immunopositive cells detected in the poorly differentiated samples all of which appeared to have a stromal/fibroblast phenotype.
There appear to be a group of poorly differentiated samples, a group of well- and well-intermediately differentiated samples, and a third group containing mainly intermediately-poorly differentiated in situ cases.
The ILVD and PLVD of medium-low differentiated specimens were significantly higher than those of the highly differentiated samples.
All samples were matched to the tissues analyzed using RNA-Seq, with the exception of the moderately differentiated samples (see Table 2).
Subsequently, we divided the sample set into one group of well/well-intermediately differentiated samples (n = 16) and a second group containing poorly/intermediately-poorly differentiated samples (n = 20).
One group contains one intermediately-poorly differentiated sample (17%) and 12 out of 14 poorly differentiated samples, and a second group all six well-differentiated samples and seven out of 10 well-intermediately differentiated samples.
One group contains 10 poorly differentiated samples and only one well-differentiated sample, whereas 83% of the well-differentiated samples group in the other, second cluster.
Following this idea, well- and well-intermediately differentiated samples may be considered to be one group, whereas poorly and intermediately-poorly differentiated samples seem to be two distinct groups of DCIS.
